1YRF
Chicken villin subdomain HP-35, N68H, pH6.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-10-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 32.155, 32.155, 56.863 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.070 |
| R-factor | 0.155 |
| R-free | 0.16100 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.110 |
| High resolution limit [Å] | 1.070 | 1.070 |
| Number of reflections | 15545 | |
| <I/σ(I)> | 50.3 | 4.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 19.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.7 | 293 | 1ul 100mg/ml peptide plus 1ul (200mM NaOAc, 2.2M AmSO4), pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.70 |
