1YRE
Hypothetical protein PA3270 from Pseudomonas aeruginosa in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-28 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.590, 92.053, 105.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.20441 |
| Rwork | 0.201 |
| R-free | 0.26451 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.894 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 43452 | |
| <I/σ(I)> | 16.7 | 2.16 |
| Completeness [%] | 96.9 | 93 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 297 | 0.2M NaCl, 0.1M TrisHCl, 25%PEG3350, 1mM AcCoA, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
