1YRC

X-ray Crystal Structure of hydrogenated Cytochrome P450cam

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.939
Spacegroup name	P 43 21 2
Unit cell lengths	63.650, 63.650, 246.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.400
R-factor	0.202
Rwork	0.202
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cpp
RMSD bond length	0.006
RMSD bond angle	1.340
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	100765
<I/σ(I)>	7.8	2.7
Completeness [%]	99.8	99.1
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.4	277	TRISHCl, KCl, Camphor, DTE, PEG 8000, pH 7.4, MICRODIALYSIS, temperature 277K