1YR0
Crystal structure of phosphinothricin acetyltransferase from agrobacterium tumefaciens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 73.849, 64.490, 145.447 |
Unit cell angles | 90.00, 99.30, 90.00 |
Refinement procedure
Resolution | 19.650 - 2.000 |
R-factor | 0.1921 |
Rwork | 0.192 |
R-free | 0.23000 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.650 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 89254 | |
<I/σ(I)> | 10.45 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 3.5M Ammonium Sulfate, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |