1YQO
T268A mutant heme domain of flavocytochrome P450 BM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9831 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.727, 152.962, 61.732 |
| Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
| Resolution | 24.000 - 1.900 |
| R-factor | 0.16842 |
| Rwork | 0.166 |
| R-free | 0.20635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2HPD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.696 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.043 | 0.144 |
| Number of reflections | 84625 | |
| <I/σ(I)> | 33.4 | 7.95 |
| Completeness [%] | 99.4 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM sodium PIPES, 40mM MgSO4, 18-21% PEG8000, pH 6.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
