1YQH
Structure of domain of unknown function DUF77 from Bacillus cereus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 115 |
Detector technology | CCD |
Collection date | 2004-08-22 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97931,0.97947 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.716, 55.175, 49.875 |
Unit cell angles | 90.00, 120.39, 90.00 |
Refinement procedure
Resolution | 43.030 - 1.700 |
R-factor | 0.16402 |
Rwork | 0.162 |
R-free | 0.20062 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.217 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.720 |
Rmerge | 0.121 | |
Number of reflections | 18421 | |
<I/σ(I)> | 5.1 | |
Completeness [%] | 92.7 | 59.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 3350, Tris-HCL, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |