1YQG
Crystal structure of a pyrroline-5-carboxylate reductase from neisseria meningitides mc58
Replaces: 1XC2Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97984, 0.97970 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 89.526, 49.845, 65.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.19727 |
| Rwork | 0.195 |
| R-free | 0.24059 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.102 | |
| Number of reflections | 26616 | |
| <I/σ(I)> | 24.66 | |
| Completeness [%] | 69.0 | 0.7 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | AMMONIUM SULFATE, HEPES, PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
