1YQF
Hypothetical protein from leishmania major unknown function sequence homologue to human p32 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Detector technology | CCD |
Collection date | 2004-11-07 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.738, 113.910, 87.858 |
Unit cell angles | 90.00, 90.49, 90.00 |
Refinement procedure
Resolution | 19.035 - 2.300 |
Rwork | 0.255 |
R-free | 0.27350 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 0.895 |
Data scaling software | ELVES |
Phasing software | EPMR (2.5) |
Refinement software | REFMAC (refmac_5.1.24 24/04/2001) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.035 |
High resolution limit [Å] | 2.300 |
Number of reflections | 56021 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.4 ul protein 19 mg/ml 0.4 ul crystallization buffer 20% PEG 4000, 100 mM CaCl2, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |