1YQC
Crystal Structure of Ureidoglycolate Hydrolase (AllA) from Escherichia coli O157:H7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9611, 0.9800, 0.9802 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.047, 74.466, 56.364 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 36.442 - 1.709 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.760 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 3.680 | 1.710 |
| Rmerge | 0.027 | 0.375 | |
| Number of reflections | 36248 | ||
| Completeness [%] | 96.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
