1YPR
SACCHAROMYCES CEREVISIAE (YEAST) PROFILIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-11 |
| Detector | SIEMENS |
| Spacegroup name | P 65 |
| Unit cell lengths | 58.100, 58.100, 151.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 * - 2.300 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ACANTHAMOEBA PROFILIN P1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.600 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 * | 0.213 * |
| Number of reflections | 12068 | |
| <I/σ(I)> | 10.75 | 2.75 |
| Completeness [%] | 93.0 | 77.5 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 1.0 M LI SULFATE, 0.1 M TRIS, PH 8.5, 0.01M NICL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | yeast profilin | 5-8 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 3.8-4.0 (M) | |
| 3 | 1 | reservoir | lithium sulfate | 1.0 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | 0.01 (M) |
