1YPR

SACCHAROMYCES CEREVISIAE (YEAST) PROFILIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-11
Detector	SIEMENS
Spacegroup name	P 65
Unit cell lengths	58.100, 58.100, 151.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 * - 2.300
R-factor	0.168
Rwork	0.168
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ACANTHAMOEBA PROFILIN P1
RMSD bond length	0.009
RMSD bond angle	25.600 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.064 *	0.213 *
Number of reflections	12068
<I/σ(I)>	10.75	2.75
Completeness [%]	93.0	77.5
Redundancy	3.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		1.0 M LI SULFATE, 0.1 M TRIS, PH 8.5, 0.01M NICL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	yeast profilin	5-8 (mg/ml)
2	1	reservoir	sodium formate	3.8-4.0 (M)
3	1	reservoir	lithium sulfate	1.0 (M)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir		0.01 (M)