1YPN

REDUCED FORM HYDROXYMETHYLBILANE SYNTHASE (K59Q MUTANT) CRYSTAL STRUCTURE AFTER 2 HOURS IN A FLOW CELL DETERMINED BY TIME-RESOLVED LAUE DIFFRACTION

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	293
Detector technology	CCD
Collection date	1995-11
Spacegroup name	P 21 21 2
Unit cell lengths	88.470, 76.150, 50.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.199
Rwork	0.199
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ah5
RMSD bond length	0.007
RMSD bond angle	22.230 *
Data reduction software	DARESBURY (LAUE SUITE)
Data scaling software	LAUENORM
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.200	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.113	0.262
Number of reflections	11383
<I/σ(I)>	4.9	2.3
Completeness [%]	72.9	69.5
Redundancy	4.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.3		pH 5.3