1YP0
Structure of the steroidogenic factor-1 ligand binding domain bound to phospholipid and a SHP peptide motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.175, 73.175, 115.731 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mLRH-1/SHP complex |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.125 | |
| Number of reflections | 51068 | |
| <I/σ(I)> | 34.8 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
