1YOX
Structure of the conserved Protein of Unknown Function PA3696 from Pseudomonas aeruginosa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-26 |
Detector | SBC-3 |
Wavelength(s) | 0.98043,0.98027,0.96517 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.912, 124.912, 164.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 |
R-factor | 0.22732 |
Rwork | 0.225 |
R-free | 0.27896 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.528 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.044 | 0.480 |
Number of reflections | 58292 | |
<I/σ(I)> | 27.03 | 2.93 |
Completeness [%] | 99.7 | 100 |
Redundancy | 8.6 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 298 | 0.5M MgCl2, 0.1M Tris pH 8.5, 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 8.50 |