1YOA
Crystal structure of a probable flavoprotein from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-10-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 132.966, 132.966, 132.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.540 - 1.900 |
R-factor | 0.2013 |
Rwork | 0.200 |
R-free | 0.22761 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.510 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.350 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 16153 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 41.7 | 43.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG400, ammonium sulfate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |