1YO2
Proton Transfer from His200 in Human Carbonic Anhydrase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.604, 41.535, 72.729 |
| Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.168 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cba |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.360 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 21751 | |
| Completeness [%] | 93.8 | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.3 | 277 | 50 mM Tris-Cl, 2.5 M AmmSO4, pH 9.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
