1YNY

Molecular Structure of D-Hydantoinase from a Bacillus sp. AR9: Evidence for mercury inhibition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	2000-11-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 64
Unit cell lengths	129.540, 129.540, 102.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.199
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modified D-hydantoinase from Thermus sp. (PDB ID 1GKP)
RMSD bond length	0.006
RMSD bond angle	1.339
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.376
Number of reflections	42440
Completeness [%]		97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	300	PEG 3350, Cesium Chloride, imidazole, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K