1YNU

Crystal structure of apple ACC synthase in complex with L-vinylglycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	C 1 2 1
Unit cell lengths	103.300, 59.360, 79.050
Unit cell angles	90.00, 124.20, 90.00

Refinement procedure

Resolution	30.000 - 2.250
Rwork	0.234
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7y
RMSD bond length	0.007
RMSD bond angle	1.220
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.063	0.277
Number of reflections	18038
<I/σ(I)>	11.6	3.2
Completeness [%]	95.5	69.7
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 4000, Tris, NiCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K