1YNT

Structure of the monomeric form of T. gondii SAG1 surface antigen bound to a human Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	C 1 2 1
Unit cell lengths	71.034, 198.285, 128.366
Unit cell angles	90.00, 89.97, 90.00

Refinement procedure

Resolution	20.000 - 3.100
Rwork	0.241
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB codes: 1F11 1kb5 1kzq
RMSD bond length	0.009
RMSD bond angle	1.535
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.190
High resolution limit [Å]	3.100	3.100
Rmerge	0.091	0.497
Number of reflections	31886
Completeness [%]	99.1	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	11	291	15% polyethylene glycol 3350, 0.25M NaCl, 150mM CAPS, pH 11, VAPOR DIFFUSION, SITTING DROP, temperature 291K