1YNM
Crystal structure of restriction endonuclease HinP1I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 117.218, 117.218, 114.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.25200 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | 0.409 |
| Number of reflections | 14790 | |
| <I/σ(I)> | 12 | 5.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 18.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | PEG 4000, sodium chloride, ammonium acetate, MES, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
