1YNF
Crystal Structure of N-Succinylarginine Dihydrolase, AstB, bound to Substrate and Product, an Enzyme from the Arginine Catabolic Pathway of Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.834, 94.172, 139.660 |
| Unit cell angles | 75.41, 78.38, 89.74 |
Refinement procedure
| Resolution | 27.320 - 1.900 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.320 |
| High resolution limit [Å] | 1.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG 10000, HEPES, pH 7.5, VAPOR DIFFUSION, temperature 293K |
