1YN9
Crystal structure of baculovirus RNA 5'-phosphatase complexed with phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9479 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.910, 74.130, 105.200 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 105.410 - 1.500 |
| R-factor | 0.16702 |
| Rwork | 0.166 |
| R-free | 0.19427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 | 0.302 |
| Number of reflections | 97263 | |
| <I/σ(I)> | 6.8 | 2.7 |
| Completeness [%] | 97.1 | 90.4 |
| Redundancy | 4.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | sodium/potassium phosphate, sodium acetate, guanidine hydrochloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
