1YN5

Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	93
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.010, 31.970, 67.980
Unit cell angles	90.00, 105.21, 90.00

Refinement procedure

Resolution	28.740 - 2.200
R-factor	0.258
Rwork	0.258
R-free	0.31700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EapH1 pdb entry 1YN4
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	9383
Completeness [%]	91.6	51.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	293	PEG 8000, ammonium sulfate, 2,6-hexanediol, cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K