1YN4
Crystal Structures of EAP Domains from Staphylococcus aureus Reveal an Unexpected Homology to Bacterial Superantigens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 93 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9199 |
Spacegroup name | P 32 |
Unit cell lengths | 38.550, 38.550, 69.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.030 - 1.800 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.23800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 10167 | |
Completeness [%] | 99.1 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | PEG 8000, zinc acetate, cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |