1YMW

The study of reductive unfolding pathways of RNase A (Y92G mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	64.334, 64.334, 63.621
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.216
Rwork	0.216
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.053	0.360
Number of reflections	23303
Completeness [%]	94.1	99.4
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	301	Ammonium sulfate, Sodium chloride, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 301K