1YMT
Mouse SF-1 LBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.03326 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.853, 73.853, 117.014 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.090 - 1.200 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1PK5 (mLRH-1 LBD) |
RMSD bond length | 0.017 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.320 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.061 | |
Number of reflections | 95280 | |
<I/σ(I)> | 38 | |
Completeness [%] | 94.0 | 100 |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 8 mg/ml mSF-1, 6X molar excess of mSHP peptide, 0.1 M Tris, 14% PEG 8000, 5% isopropanol, 15% xylitol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |