1YMK

Crystal Structure of the CDC25B phosphatase catalytic domain in the apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	50.237, 70.995, 73.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.770 - 1.700
R-factor	0.18
Rwork	0.178
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qb0
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	29.770
High resolution limit [Å]	1.700
Number of reflections	29361

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.25		DTT,Tris, Ammonium Sulfate, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 100.0K