1YM8
crystal structure of GZZ shows up puckering of the proline ring in the Xaa position.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 23.230, 28.580, 80.510 |
Unit cell angles | 90.00, 97.85, 90.00 |
Refinement procedure
Resolution | 39.880 - 1.550 |
R-factor | 0.2351 |
Rwork | 0.235 |
R-free | 0.25600 |
Structure solution method | MIR |
Starting model (for MR) | 1k6f |
RMSD bond length | 0.007 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.880 | 1.650 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 15444 | |
Completeness [%] | 100.0 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | peg 400, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |