1YM3
Crystal Structure of carbonic anhydrase RV3588c from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.085 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 56.377, 56.377, 104.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.200 - 1.750 |
| R-factor | 0.18134 |
| Rwork | 0.179 |
| R-free | 0.22946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1I6O and 1DDZ |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.200 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 13934 | |
| <I/σ(I)> | 23.35 | 5 |
| Completeness [%] | 83.3 | 80.1 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | MgCl2, PEG 400, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
