1YLQ
Crystal structure of putative nucleotidyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.350, 38.350, 244.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.016 |
| R-factor | 0.2166 |
| Rwork | 0.217 |
| R-free | 0.26746 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14806 | |
| Completeness [%] | 97.9 | 79.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 297 | PEG3350, Ammonium Sulphate,B-Tris, pH 5.5, VAPOR DIFFUSION, temperature 297K |
| 1 | VAPOR DIFFUSION | 5.5 | 297 | PEG3350, Ammonium Sulphate,B-Tris, pH 5.5, VAPOR DIFFUSION, temperature 297K |
| 1 | VAPOR DIFFUSION | 5.5 | 297 | PEG3350, Ammonium Sulphate,B-Tris, pH 5.5, VAPOR DIFFUSION, temperature 297K |
