1YLE
The structure of arginine/ornithine succinyltransferase subunit AI from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-10-10 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97799,0.97944,0.97961 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.295, 73.295, 110.786 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.740 - 1.700 |
| R-factor | 0.19155 |
| Rwork | 0.190 |
| R-free | 0.22195 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.199 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.740 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.442 | |
| Number of reflections | 38436 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Bis-Tris, magnesium formate, sodium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
