1YL6
crystal structure of Mycobacterium tuberculosis dihydrodipicolinate reductase (Rv2773c) (crystal form B)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-15 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 87.130, 89.460, 77.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.20415 |
Rwork | 0.199 |
R-free | 0.25689 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9l |
RMSD bond length | 0.015 |
RMSD bond angle | 1.689 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.061 | 0.494 |
Number of reflections | 13831 | |
<I/σ(I)> | 23.9 | 4 |
Completeness [%] | 98.9 | 99.8 |
Redundancy | 31.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 100 mM Tris/HCl, 24 % PEG 3350, 140 mM MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |