1YL1
Effect of alcohols on protein hydration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 2004-05-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.288, 79.288, 37.957 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.230 - 1.900 |
Rwork | 0.207 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2lym |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.230 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.116 |
Number of reflections | 9987 | |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | sodium chloride, sodium acetate bufferbuffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |