1YKW
Crystal Structure of a Novel RuBisCO-Like Protein from the Green Sulfur Bacterium Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.348, 78.451, 90.369 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 87.710 - 2.000 |
| R-factor | 0.20329 |
| Rwork | 0.201 |
| R-free | 0.24539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5rub |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.626 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 60951 | |
| <I/σ(I)> | 7.7 | 2 |
| Completeness [%] | 97.5 | 84.7 |
| Redundancy | 3.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | PEG 3350, magnesium formate , pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
