1YKS
Crystal structure of yellow fever virus NS3 helicase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793, 0.9797, 0.9796, 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.018, 43.801, 93.069 |
| Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.193 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 39232 | |
| Completeness [%] | 97.6 | 93.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 10000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 10000, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
