1YK3
Crystal structure of Rv1347c from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.665, 77.261, 296.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.960 - 2.200 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.25800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.250 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.092 | 0.551 |
Number of reflections | 96453 | |
<I/σ(I)> | 21.2 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Methoxy, PEG 5000, Tris-HCl, beta-octylglucoside, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | Methoxy, PEG 5000, Tris-HCl, beta-octylglucoside, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |