1YJH
Crystal Structure of Cimex Nitrophorin Ferrous NO Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.000, 41.560, 65.370 |
| Unit cell angles | 90.00, 95.29, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.2054 |
| Rwork | 0.203 |
| R-free | 0.24992 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ntf |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.918 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.036 | 0.186 |
| Number of reflections | 30416 | |
| <I/σ(I)> | 16.3 | 4.2 |
| Completeness [%] | 95.5 | 83.5 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 4000, Li2SO4, ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
