1YJE
Crystal structure of the rNGFI-B ligand-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00003 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 90.422, 90.422, 140.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ebtry 1PDU |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.521 |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 11676 | |
| <I/σ(I)> | 20.8 | 5.1 |
| Completeness [%] | 98.3 | 97.1 |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | NaCl, BisTris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
