1YJB
SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) (MUTANT WITH MET 50 REPLACED BY PHE, ASN 76 REPLACED BY ASP, GLY 169 REPLACED BY ALA, GLN 206 REPLACED BY CYS, ASN 218 REPLACED BY SER AND LYS 256 REPLACED BY TYR) (M50F, N76D, G169A, Q206C, N218S, AND K256Y) IN 35% DIMETHYLFORMAMIDE
Experimental procedure
| Detector technology | DIFFRACTOMETER |
| Collection date | 1995-07-15 |
| Detector | RIGAKU AFC-6R |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.670, 79.620, 37.230 |
| Unit cell angles | 90.00, 114.27, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.800 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.713 |
| Data reduction software | TEXSAN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.073 |
| Total number of observations | 21228 * |
| Number of reflections | 10660 * |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 5 (mM) | |
| 3 | 1 | drop | PEG3400 | 8.5 (%(w/v)) | |
| 4 | 1 | drop | 2-propanol | 8.5 (%(v/v)) | |
| 5 | 1 | drop | sodium citrate | 50 (mM) | |
| 6 | 1 | reservoir | PEG3400 | 17 (%(w/v)) | |
| 7 | 1 | reservoir | 2-propanol | 17 (%(v/v)) | |
| 8 | 1 | reservoir | sodium citrate | 100 (mM) |
