1YJ8

Initial structural analysis of Plasmodium falciparum Glycerol-3-phosphate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-3
Synchrotron site	SSRL
Beamline	BL11-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-11
Detector	ADSC QUANTUM 210
Spacegroup name	P 63 2 2
Unit cell lengths	177.455, 177.455, 232.699
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.540 - 2.850
R-factor	0.23706
Rwork	0.236
R-free	0.25576
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.944
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	50574
<I/σ(I)>	8.4	1.8
Completeness [%]	99.3	96.3
Redundancy	5.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	293	PEG 400, Potassium phosphate monobasic, CAPS, DTT, pH 10, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	10	293	PEG 400, Potassium phosphate monobasic, CAPS, DTT, pH 10, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	10	293	PEG 400, Potassium phosphate monobasic, CAPS, DTT, pH 10, VAPOR DIFFUSION, SITTING DROP, temperature 293K