1YII
Crystal Structures of Chicken Annexin V in Complex with Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2002-09-02 |
| Wavelength(s) | 1.040249 |
| Spacegroup name | H 3 |
| Unit cell lengths | 98.110, 98.110, 94.939 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.660 - 1.420 |
| R-factor | 0.1941 |
| Rwork | 0.194 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.660 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.041 | 0.332 |
| Number of reflections | 62969 | |
| <I/σ(I)> | 20 | 1.8 |
| Completeness [%] | 97.9 | 87.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | Ammonium Sulfate, calcium chloride, HEPES, pH 8.0, VAPOR DIFFUSION, temperature 298K |
