1YI8

Crystal structure of tryptophanyl trRNA synthetase II from Deinococcus radiodurans in complex with L-Trp

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	193
Detector technology	CCD
Collection date	2004-03-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978, 0.979, 0.92
Spacegroup name	C 1 2 1
Unit cell lengths	211.800, 58.500, 88.900
Unit cell angles	90.00, 100.72, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.26
Rwork	0.260
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.100	2.100
Rmerge	0.076	0.182
Number of reflections	72160
<I/σ(I)>	20.4	2.7
Completeness [%]	80.0	80
Redundancy	2.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG4K, 0.2M DiAMMONIUM HYDROGEN PHOSPHATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG4K, 0.2M DiAMMONIUM HYDROGEN PHOSPHATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG4K, 0.2M DiAMMONIUM HYDROGEN PHOSPHATE, VAPOR DIFFUSION, HANGING DROP, temperature 295K