1YI3
Crystal Structure of Pim-1 bound to LY294002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.647, 97.647, 80.725 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.460 - 2.500 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.470 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.336 |
| Number of reflections | 14445 | |
| <I/σ(I)> | 12 | 2.6 |
| Completeness [%] | 94.9 | 87.6 |
| Redundancy | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | ammonium phosphate, citrate buffer, NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
