1YHT
Crystal structure analysis of Dispersin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91837, 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.023, 86.129, 185.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.760 - 2.000 |
| R-factor | 0.15421 |
| Rwork | 0.151 |
| R-free | 0.21087 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.623 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.760 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.193 |
| Number of reflections | 21412 | |
| <I/σ(I)> | 15.6 | 7.1 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 10000, ammonium acetate, BisTris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 10000, ammonium acetate, BisTris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
