1YHK
Trypanosoma cruzi farnesyl diphosphate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.925, 57.925, 397.697 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.940 - 2.100 |
| R-factor | 0.17581 |
| Rwork | 0.173 |
| R-free | 0.22686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Selenomethionine replaced protein model (MAD) |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 65.940 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 21828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 4.6 | 293 | pH 4.6, LIQUID DIFFUSION, temperature 293K |
