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1YHH

Uncyclized precursor structure of S65A Y66S G67A GFP variant

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-1
Synchrotron siteSSRL
BeamlineBL9-1
Temperature [K]100
Detector technologyCCD
Collection date2004-04-19
DetectorADSC QUANTUM 315
Wavelength(s)0.97975
Spacegroup nameP 21 21 21
Unit cell lengths51.260, 62.705, 70.241
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 1.500
R-factor0.217
Rwork0.217
R-free0.22800
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.011
RMSD bond angle1.600
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.550
High resolution limit [Å]1.5001.500
Rmerge0.0690.359
Number of reflections35794
<I/σ(I)>21.1
Completeness [%]96.997.5
Redundancy4.13.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8298PEG4000, 50 mM MgCl2, 50 mM Hepes , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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