1YHH
Uncyclized precursor structure of S65A Y66S G67A GFP variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97975 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.260, 62.705, 70.241 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.500 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.069 | 0.359 |
Number of reflections | 35794 | |
<I/σ(I)> | 21.1 | |
Completeness [%] | 96.9 | 97.5 |
Redundancy | 4.1 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG4000, 50 mM MgCl2, 50 mM Hepes , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |