1YHG
Uncyclized precursor structure of S65G Y66S V68G GFP variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.301, 71.098, 60.940 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.119 | 0.312 |
| Number of reflections | 13380 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 4000, 50 mM MgCl2, 50 mM Hepes 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
