1YHF
Crystal Structure of Conserved SPY1581 Protein of Unknown Function from Streptococcus pyogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-02 |
Detector | SBC-3 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 39.897, 39.897, 144.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 2.000 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.24800 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.434 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.137 | 0.746 |
Number of reflections | 8535 | |
<I/σ(I)> | 33.6 | 2.62 |
Completeness [%] | 99.1 | 94.8 |
Redundancy | 23.4 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | 30% PEG 3000, 0.1 M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 4.50 |