1YGT
Dynein Light Chain TcTex-1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.99187, 0.97950, 0.97897, 0.96672 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.598, 60.598, 48.485 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 6.000 - 1.700 |
R-factor | 0.2 |
Rwork | 0.193 |
R-free | 0.23500 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 0.033 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.198 |
Number of reflections | 10932 | |
<I/σ(I)> | 37.6 | 12 |
Completeness [%] | 93.8 | 96.9 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9.5 | 293 | 0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K |
1 | VAPOR DIFFUSION | 9.5 | 293 | 0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K |
1 | VAPOR DIFFUSION | 9.5 | 293 | 0.1M CHES, pH 9.5, 2.4 M (NH4)2SO4 and 0.1M Li2SO4, vapor diffusion, temperature 293K |