1YGS
CRYSTAL STRUCTURE OF THE SMAD4 TUMOR SUPPRESSOR C-TERMINAL DOMAIN
Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1997-07-04 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 197.842, 197.842, 197.842 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.100 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.27900 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.538 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SQUASH |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.065 * | |
Total number of observations | 30691 * | |
Number of reflections | 18650 | |
<I/σ(I)> | 32 | 9.9 |
Completeness [%] | 93.5 | 93.1 |
Redundancy | 5.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | 100 MM NAMES, PH6.5, 25% PEG MONOMETHYLETHER 5000, 5 MM DTT, 200 MM (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | reservoir | MES | 100 (mM) | |
3 | 1 | reservoir | mPEG5000 | 25 (%) | |
4 | 1 | reservoir | ammonium sulfate | 200 (mM) | pH6.5 |