1YGP

PHOSPHORYLATED FORM OF YEAST GLYCOGEN PHOSPHORYLASE WITH PHOSPHATE BOUND IN THE ACTIVE SITE.

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-04-08
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	103.910, 143.930, 169.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.179
Rwork	0.179
R-free	0.23900
RMSD bond length	0.018
RMSD bond angle	2.870
Data reduction software	R-AXIS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.105
Total number of observations	247322 *
Number of reflections	50707
Completeness [%]	72.0
Redundancy	5	0.043 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	18 *	used to seeding, Lin, K., (1997) Structure, 5, 1511. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	32 (mg/ml)
10	1	reservoir	Bis-Tris	100 (mM)
11	1	reservoir		50 (mM)
12	1	reservoir	Glc-1-P	50 (mM)
13	1	reservoir	ammonium sulfate	400 (mM)
14	1	reservoir	PEG4000	4.5 (%)
15	1	drop	reservoir		equal volume of drop solution
2	1	drop	sodium succinate	130 (mM)
3	1	drop	DTT	2 (mM)
4	1	drop	EDTA	0.2 (mM)
5	1	drop	leupeptin	0.001 (mg/ml)
6	1	drop	aprotonin	0.001 (mg/ml)
7	1	drop	pepstatin A	0.001 (mg/ml)
8	1	drop	PMSF	0.5 (mM)
9	1	drop	sodium azide	0.02 (%)